Tag Archives: using

Dissecting direct reprogramming from fibroblast to neuron using single-cell RNA-seq

Direct lineage reprogramming represents a remarkable conversion of cellular and transcriptome states. However, the intermediate stages through which individual cells progress during reprogramming are largely undefined. Here we use single-cell RNA sequencing at multiple time points to dissect direct reprogramming from mouse embryonic fibroblasts to induced neuronal cells. By deconstructing heterogeneity at each time point and ordering cells by transcriptome similarity, we find that...

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Efficient introduction of specific homozygous and heterozygous mutations using CRISPR/Cas9

The bacterial CRISPR/Cas9 system allows sequence-specific gene editing in many organisms and holds promise as a tool to generate models of human diseases, for example, in human pluripotent stem cells. CRISPR/Cas9 introduces targeted double-stranded breaks (DSBs) with high efficiency, which are typically repaired by non-homologous end-joining (NHEJ) resulting in nonspecific insertions, deletions or other mutations (indels). DSBs may also be repaired by homology-directed repair...

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Using the uncertainty principle against itself to gain precision

You can't beat the Heisenberg limit, but with enough math, you can come close. (credit: Focus Features)

Accurate measurement underlies a huge amount of modern technology. Atomic clocks, fiber optical communications systems, and many other types of hardware require accurate and precise measurements. The laws of quantum mechanics, on the other hand, are designed to annoy anyone...

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